![]() ![]() Ma’arif B, Abada I, Mahardiani A, Hakim A, Maulina N, Purwitasari N, Hidayah K, Jing S. The western blot method has selectivity for detecting complex-level protein expression.Ĭost Efficiency ELISA Immunocytochemistry Selectivity Sensitivity, Processing Time Western Blotĭownload this article as: Copy the following to cite this article: The immunocytochemistry method has a higher level of sensitivity and a faster processing time, whereas the ELISA method has a higher level of selectivity and less cost efficiency. ![]() Comparison of immunocytochemistry and ELISA methods in order to analyze the activities of ALP in osteoblast induced by genistein includes selectivity, sensitivity, processing time, and cost efficiency parameters. 24 relevant research articles were uncovered as a result of this systematic review. Articles with the keywords "genistein or osteoblast cells or alkaline phosphate or immunocytochemistry or immunofluorescence or ELISA or western blot" were retrieved from databases including Google Scholar, PubMed, Researchgate, and Sciencedirect. Using the PRISMA guideline technique, choose and strategize article searches by reading the title, abstract, and then the whole text of the article. ![]() Genistein induction can stimulate bone formation and promote the increase of alkaline phosphate (ALP) activities in osteoblast cells which can be observed by immunocytochemistry or Enzyme-linked Immunosorbent Assay (ELISA) or Western blot method. Genistein is a phytoestrogen compound in the isoflavone group containing a similar structure compared to 17β-estradiol, thus it can bind to estrogen receptors and produce an estrogenic effect. One of the causes of osteoporosis is estrogen deficiency. Osteoporosis is a bone disorder characterized by the decrease of bone mass along with bone micro-architecture damage and has a risk become a fracture. Burhan Ma’arif 1, Iffatul Abada 1, Anisah Mahardiani 1, Abdul Hakim 1*, Novia Maulina 1, Neny Purwitasari 2, Khoirul Hidayah 3 and Seow Lay Jing 4ġDepartment of Pharmacy, Faculty of Medical and Health Science, Maulana Malik Ibrahim State Islamic University, Malang, IndonesiaĢDepartment of Pharmaceutical Science, Faculty of Pharmacy, Universitas Airlangga, Surabaya, IndonesiaģFaculty of Sharia, Maulana Malik Ibrahim State Islamic University, Malang, IndonesiaĤDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy and Health Sciences, Universiti Kuala Lumpur Royal College of Medicine, Perak, Malaysia ![]()
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